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How have the structure and function of microorganisms been revealed by the use of microscopy?

Topic

Cell Structure and Function

Framing Question

How have the structure and function of microorganisms been revealed by the use of microscopy?

Learning Objectives

  • List three different bacterial cell morphologies.
  • Identify microbial structures from a given image.
  • Describe how the cell structure of Gram-negative and Gram-positive cells leads to a given Gram stain result.
  • Explain how bright-field microscopy works and why specimens must be stained.
  • Explain how phase-contrast microscopy works and why specimens do not need to be stained.
  • Decide on the correct type of microscopy and sample preparation for a given situation.
  • Explain how magnification and resolution are controlled in a microscope.
  • State the advantages and disadvantages of using bright-field, phase-contrast, dark-field, fluorescence, confocal scanning laser, transmission electron, and scanning electron microscopy for a given situation.

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