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What experimental methods are commonly used to analyze gene structure and gene expression?

Topic

Methods & Tools in Genetics

Framing Question

What experimental methods are commonly used to analyze gene structure and gene expression?

Learning Objectives

  • Explain reverse genetics and compare methods for generating specific mutations in the genome vs. generating phenocopies using techniques such as RNAi or morpholinos.
  • Explain the method of SNP/STR mapping and interpret SNP/STR mapping data to pinpoint the chromosomal location of a human disease gene.
  • Interpret complementation tests, including an assessment of the molecular interactions that might yield the results observed.

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