Macromolecular Structure Determines Function and Regulation
What factors determine structure?
- Recognize the repeating units in biological macromolecules and be able to discuss the structural impacts of the covalent and non-covalent interactions involved.
- Discuss the composition, evolutionary change and hence structural diversity of the various types of biological macromolecules found in organisms.
- Discuss the chemical and physical relationships between composition and structure of macromolecules.
- Compare and contrast the primary, secondary, tertiary and quaternary structures of proteins and nucleic acids.
- Use various bioinformatics approaches to analyze macromolecular primary sequence and structure.
- Compare and contrast the effects of chemical modification of specific amino acids on a three dimensional structure of a protein.
- Compare and contrast the ways in which a particular macromolecule might take on new functions through evolutionary changes.
- Use various bioinformatics and computational approaches to compare primary sequences and identify the impact of conservation and/or evolutionary change on the structure and function of macromolecules.
- Predict the effects of mutations on the activity, structure or stability of a protein and design appropriate experiments to assess the effects of mutations.
- Propose appropriate chemical or chemical biology approaches to explore the localization and interactions of biological macromolecules.
- Discuss how mutations of a duplicated gene generate functional diversity.
- Evaluate chemical and energetic contributions to the appropriate levels of structure of the macromolecule and predict the effects of specific alterations of structure on the dynamic properties of the molecule.